Each network laboratory uses prepared mixtures of purified Hemoglobin A1c and HbA0 as calibrators. The relationship between HbA1c results from the NGSP network (%HbA1c) and the IFCC network (mmol/mol) has been evaluated and a master equation has been developed (NGSP = [0.09148 * IFCC] + 2.152). This relationship will continue to be monitored.. The relationship between the IFCC and NGSP (both in %HbA1c) is shown in Figure 1. IFCC results are consistently 1.5-2% HbA1c lower throughout the range of values. The relationship between the NGSP network and IFCC networks was evaluated and a master equation was developed to document this relationship (NGSP = [0.9148 * IFCC] + 2.152).

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Due to its link to the DCCT and UKPDS, the NGSP system was, by an overwhelming margin, the most popular global HbA 1c standardization system. NGSP-certified methods are currently used worldwide in the clinical laboratories that measure patient samples, and these results are directly traceable to the DCCT and UKPDS.. Glycated hemoglobin concentrations (most commonly hemoglobin A1C; HbA 1c) reflect time-averaged blood glucose during the previous 2-3 months and are used as the gold standard for long-term follow-up of glycemic control.Standardization with common calibration was first proposed in 1984 ().It was only after the publication of the Diabetes Control and Complications Trial (DCCT) study in 1993.